. Author manuscript; available in PMC: 2021 Jun 17.

Published in final edited form as: J Agric Food Chem. 2020 Jun 4;68(24):6576–6587. doi: 10.1021/acs.jafc.0c01475

Table 1.

IC₅₀ Values (in µg/mL) of Anti-proliferation Activities of ME, its Fractions and Key Active Components (MGN, HNK, MHNK), Individually and in Different Ratios in Oral Cancer Cell Lines (Cal-27 and SCC-9) Based on the MTT Assay Run in Triplicate.

Cell lines	Reagent	Treatment period (hr)
Cell lines	Reagent	24	48	72	96
*Cal-27*
	ME^a	36.3 ± 2.6	25.7 ± 2.3	14.2 ± 2.5	8.2 ± 1.9
	MGN	20.7 ± 1.8^*	11.9 ± 0.6^*	5.1 ± 0.6^*	4.0 ± 0.8^*
	HNK	20.8 ± 1.3^*	12.2 ± 1.0^*	6.6 ± 0.6^*	4.2 ± 0.4^*
	MHNK	14.5 ± 0.9^*	7.7 ± 0.8^*	5.6 ± 1.4^*	3.2 ± 0.3^*
	Fraction 1			>100^*
	Fraction 2			72.1 ± 2.3^*
	Fraction 3			61.8 ± 9.4^*
	Fraction A^b			22.5 ± 1.4
	1:1:1 (MGN:HNK:MHNK)			4.8 ± 0.2^*
	1:2:6 (MGN:HNK:MHNK)			3.8 ± 0.2^*
	1:2:3 (MGN:HNK:MHNK)			3.9 ± 0.3^*
	5-FU (positive control)			0.4 ± 0.1^*

*SCC-9*
	ME^a			14.4 ± 2.9
	MGN			7.9 ± 0.4^*
	HNK			5.5 ± 0.6^*
	MHNK			5.2 ± 1.3^*
	Fraction 1			> 100^*
	Fraction 2			> 100^*
	Fraction 3			99.5 ± 0.6^*
	Fraction A^b			19.6 ± 1.7
	5-FU (positive control)			1.9 ± 0.4^*

Ratio of three key active ingredients in ME was 1.0 : 1.4 : 1.2 (MGN : HNK : MHNK)

Ratio of compounds in Fraction A was 1.0 : 0.82 : 0.28 (MGN : HNK : MHNK)

indicates significant different with the respective the respective IC₅₀ value of ME. (p < 0.05). Statistical analysis was performed using one-way ANOVA with Tukey similarity test.