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. 2020 Nov 1;10:198. doi: 10.1186/s13568-020-01134-z

Table 3.

Strain characteristics potentially involved in pathogenicity on plants

Strainsa Taxonomic position Pathogenicity on lemonc Pathogenicity on cantaloupee HRf Toxin produc-tiong INAh
Necrosis diameter after injection in fruit(cm) Necrosis diameter after spraying fruit (cm) Necrosis surface after infiltration of leaves (cm2) Mean symptom rate F. of plants with rate > 1 Agressivness HR ontobacco Inhibition zone (cm) Syringomycinproduction Freezing Test (°C) Ice nucleationactivity
BE12A P. congelans(PG02c) 0.1 ± 0.1 0.0 0 3.3 1.0  +   ±  0.6  +  − 1.5  + 
BE1 P. syringae (PG02b) 1.7 ± 0.4 0.8 ± 0.5 0.9 3.6 1.0  +   +  1.1  +  − 1.5  + 
BE3 P. syringae (PG02b) 3.2 ± 0.4 1.0 ± 0.3 1.2 3.5 1.0  +   +  1.1  +  − 2.1  + 
ly3DA P. syringae (PG02b) 1.9 ± 0.2 0.2 ± 0.1 1.0 3.6 1.0  +   +  1.3  +  − 2.6  + 
ly3EA P. syringae (PG02b) 3.9 ± 0.4 1.2 ± 0.2 1.1 2.3 1.0  +   +  1.2  +  − 1.5  + 
ly3GB P. syringae (PG02b) 2.7 ± 0.1 0.8 ± 0.1 1.3 2.5 0.9  +   +  1.3  +  − 2.6  + 
ly5HA P. syringae (PG02b) 2.1 ± 1.0 0.7 ± 0.2 1.0 3.8 1.0  +   +  1.2  +  − 1.5  + 
lyGA P. syringae (PG02b) 2.6 ± 0.1 0.8 ± 0.2 1.0 2.8 1.0  +   +  1.2  +  − 1.4  + 
lyGC P. syringae (PG02b) 3.0 ± 0.1 1.2 ± 0 0.9 0.0 0.0 −  +  1.2  +  − 1.5  + 
KB49 P. syringae (PG02b) 2.3 ± 0.4 1.4 ± 0.3 1.0 3.5 1.0  +   +  1.1  +  − 2.5  + 
KC19 P. syringae(PG02b) 2.8 ± 0.4 1.6 ± 0.2 1.0 3.3 1.0  +   +  1.2  +  − 1.9  + 
KC29B2 P. syringae(PG02b) 3.3 ± 0.4 0.9 ± 0.1 1.1 3.1 1.0  +   +  1.3  +  − 1.7  + 
KC46 P. syringae (PG02b) 3.3 ± 0.3 0.0 0.8 3.7 1.0  +   +  1.2  +  − 2.2  + 
KC54 P. syringae (PG02b) 3.4 ± 0.2 1.1 ± 0.4 1.1 3.8 1.0  +   +  1.1  +  − 1.2  + 
KC55 P. syringae (PG02b) 3.5 ± 0.8 1.6 ± 0.2 0.9 2.8 0.8  +   +  1.3  +  − 1.4  + 
KC82 P. syringae (PG02b) 3.8 ± 0.4 0.5 ± 01 1.0 3.7 1.0  +   +  1.6  +  − 1.6  + 
TRR12 P. syringae (PG02b) 2.5 ± 0.4 0.0 1.0 2.7 1.0  +   +  1.3  +  − 1.4  + 
TRR15 P. syringae (PG02b) 2.5 ± 0.3 0.7 ± 0.1 1.2 2.8 0.9  +   +  1.3  +  − 2.4  + 
TRR9 P. syringae (PG02b) 2.7 ± 0.2 0.3 ± 0.4 0.9 2.8 1.0  +   +  1.4  +  − 1.6  + 
E10AA P. syringae (PG02b) 1.8 ± 0.3 1.2 ± 0.1 1.5 3.2 1.0  +   +  1  +  − 1.3  + 
E10CA P. syringae (PG02b) 2.8 ± 0.2 0.1 ± 0.1 0.8 3.0 1.0  +   +  0.7  +  − 2.0  + 
E10CB2 P. syringae(PG02b) 1.8 ± 0.2 0.6 ± 0.3 1.2 3.5 1.0  +   +  1.2  +  − 1.8  + 
E11 P. syringae (PG02b) 2.7 ± 0.6 1.4 ± 0.1 1.1 2.3 0.6  +   +  1.2  +  − 2.1  + 
E12A P. syringae (PG02b) 2.6 ± 0.1 2.5 ± 0.3 1.2 3.2 1.0  +   +  1.1  +  − 1.6  + 
E91 P. syringae (PG02b) 3.0 ± 0.4 1.9 ± 0.4 1.0 3.8 1.0  +   +  1.1  +  − 1.8  + 
E93A P. syringae (PG02b) 2.0 ± 0.4 0.5 ± 0.2 1.1 3.0 0.8  +   +  0.9  +  − 2.3  + 
E9A P. syringae (PG02b) 3.0 ± 0.3 2.2 ± 0.3 0.7 3.7 1.0  +   +  1.5  +  − 1.2  + 
EL1A P. syringae (PG02b) 2.2 ± 0.3 0.0 1.0 2.9 0.9  +   +  0.7  +  −1.2  + 
EL2 P. syringae (PG02b) 4.1 ± 0.2 0.8 ± 0.3 0.8 2.7 0.9  +   +  0.6  +  − 0.9  + 
M5* P. syringae (PG02b) 1.5 ± 0.1 ND ND ND ND ND ND ND ND ND ND
M47* P. syringae (PG02b) 1.6 ± 0.4 ND ND ND ND ND ND ND ND ND ND
V2A2F14* P. cerasi(PG02a) 1.1 ± 0.3 ND ND 2.5 0.9  +  − 0.0 − ND ND
V2A2F15* P. cerasi(PG02a) ND ND ND ND ND ND − 0.0 − ND ND
V2A2F27* P. cerasi(PG02a) 1.2 ± 0.1 ND ND 2.3 0.9  +  − 0.2  ±  ND ND
V2A2F45* P. cerasi(PG02a) 1.3 ± 0.4 ND ND 0.0 0 − − 1.5  +  ND ND
V2A2F52* P. cerasi(PG02a) ND ND ND 0.1 0.1 − − 0.0 − ND ND
V2A2F79* P. cerasi(PG02a) ND ND ND 0.0 0.0 − − 0.0 − ND ND
CC94b P. syringae(PG02d) ND ND ND 2.7 1.0  +   +  1.2  +  − 3.2  + 
Waterb − 0 0 0 0.0 0 − − 0 −  > 10 −

a Strainwith * were lost during the study and were no longer available to do all tests

b CC94: positive control. Water: negative control

c Necrotic diameters on fruits were measured in cm, lesion areas on leaves were expressed in cm2 (average of three replicates ± standard deviation)

d ND, not determined

e Symptom intensity on cantaloupe was rated on 12 seedlingsfrom 0 (no symptom) to 4 (dead plantlet) seven days after inoculation with 106 cells. F. of plants with rate > 1: Frequency of plants with rate > 1

f HR, Hypersensitive Response of tobacco after infiltration of a leaf with 106 bacteria. The reading was done 48 h after infiltrationg Production of syringomycine-like toxins was tested on agar medium usingGeotrichumalbicansas a test organism

h INA, Ice Nucleation Activity was tested with bacterial suspension at 106cells/ml