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. 2020 Oct 19;11:570903. doi: 10.3389/fmicb.2020.570903

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Copyright © 2020 Hu, He, Elsheikha, Zou, Ehsan, Ma, Zhu and Cong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of T. gondii B1 gene amplified by specific semi-nested PCR in infected and uninfected mouse brain tissues. The amplified band of 98 bp was detected in infected mouse brain (11 and 33 dpi). However, no product was detected in the brain tissues of uninfected mice at the same time points. Lane M: 500 bp DNA molecular ladder; Lane P: positive control; Lane N: negative control; Lanes 1–3: infected brain tissues at 11 dpi; Lanes 4–6: infected brain tissues at 33 dpi; Lanes 7–9: uninfected brain tissues at 11 dpi; Lanes 10–12: uninfected brain tissues at 33 dpi.