Table 4.
Effects of СhBac3.4 variants containing additional RFR-motif on biofilm formation.
| Minimal concentrations of peptides required for exerting the specified effectagainst the specified bacteria | ||||||
|---|---|---|---|---|---|---|
| ChBac3.4-NH2 | RFR-ChBac3.4-1-NH2 | RFR-ChBac3.4(1-14)-NH2 | ||||
| Effect | A. baumannii 7226/16 | P. aeruginosa MDR 522/17 | A. baumannii 7226/16 | P. aeruginosa MDR 522/17 | A. baumannii 7226/16 | P. aeruginosa MDR 522/17 |
| 100% inhibition of biofilm formation a | 2×MIC | MIC | MIC | 2×MIC | 4×MIC | 4×MIC |
| >50% decrease in the thickness of the formed biofilm | ½ MIC | 1∕64 MIC | ¼ MIC | ½ MIC | 2×MIC | MIC |
| Statistically significant decrease in thickness of the formed biofilm b | 1∕16 MIC | 1∕128 MIC | ¼ MIC | 1∕1024 MIC | 2×MIC | 1∕512 MIC |
MIC, minimal inhibitory concentration (see Table 2 ).
a
No difference found by Mann–Whitney U-test (p < 0.05) between the sample containing AMP and the negative control of biofilm formation (growth medium without bacteria).
b
A difference found by Mann–Whitney U-test (p < 0.05) between the sample containing AMP and the positive control of biofilm formation (bacteria without antimicrobial compounds in growth medium).