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. 2020 Oct 19;8:566152. doi: 10.3389/fcell.2020.566152

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Copyright © 2020 Fiorentino, Parrilli, Garagna and Zuccotti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative 1.5 μm/pixel microCT image of the mouse ovary. (A) Schematic representation of the eight main stages of the mouse folliculogenesis, with their corresponding size in diameter (mean ± s.d.), during the pre-antral (T1–T5) and antral (T6–T8) growth phases. (B) A representative microCT section highlighting follicles from T4 to T8 and corpora lutea (CL). On the right-hand side (top), an enlargement of a secondary T4 follicle (arrow); on the right-hand side (bottom), an enlargement of a fully grown T8 follicle, in which the different cytological components (granulosa cells, antrum, cumulus cells, zona pellucida space, oocyte with its nucleus) are clearly visible. (C) microCT section with an enlargement of a vessel (arrowhead; 150 μm in diameter) at the ovarian hilum site. (D) microCT section with an enlargement of a group of small vessels (arrowheads; 35 μm in diameter) in the medulla region. Bar, 500 μm.