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. 2020 Oct 19;8:569729. doi: 10.3389/fcell.2020.569729

FIGURE 1.

FIGURE 1

Differential expression of CXCR4 in membrane cells and total receptors in MDA-MB-231 and MCF-10A. Recombinant protein (rP21) is not cytotoxic and binds in cells. Evaluation of CXCR4 levels by confocal microscopy (A) and flow cytometry (B). MCF-10A (C) and MDA-MB-231 (D) were treated with rP21 at different concentrations (100, 50, 25, 12.5 and 6.25 μg/mL) and did not exhibit alterations in cell viability. These data are from one experiment representative of three independent experiments. The cells were incubated for 1 hour with rP21 (100 μg/mL). rP21 labeling by flow cytometry showed protein binding in the MCF-10 A (E) and MDA-MB-231 (F) cells. These results are representative of at least three independent experiments. Data show the mean ± SEM. Significant differences were determined using student t-tests and one-way ANOVA. Differences were considered significant when p < 0.05. **p = 0.0025, ***p = 0.0005, and ****p < 0.0001.