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. 2020 Oct 19;8:569729. doi: 10.3389/fcell.2020.569729

FIGURE 4.

FIGURE 4

The rP21 protein downregulated CXCR4 gene expression, decreased the CXCR4 levels, and is internalized in MDA-MB-231. Gene expression of CXCR4 in MCF-10A (A) and MDA-MB-231 (B) after 72 h of treatment. Evaluation of CXCR4 in a membrane cell after rP21 treatment for 1 h in MCF-10A (C) and MDA-MB-231 (D) cells. Evaluation of CXCR4 in the cytoplasm after rP21 treatment for 1 and 72 h in MCF-10A (E) and MDA-MB-231 (F) cells. MCF-10A (G) and MDA-MB-231 (H) cells were treated with rP21 (100 μg/ml) for 1 and 24 h, fixed, permeabilized, rP21 (green) and CXCR4 (red) stained, and analyzed by confocal microscopy. Blue, cell nuclei. White arrows indicate the location of rP21. Bars, 20 μm represents × 63 objective. These results are representative of at least three independent experiments. Data show the mean ± SEM. Significant differences were determined using t-test. Differences were considered significant when p < 0.05. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.