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. 2020 Oct 19;8:565261. doi: 10.3389/fcell.2020.565261

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Copyright © 2020 Zhong, Li, Chen, Diao, He, Zhang, Zhang, Yuan and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bioinformatic prediction and functional analysis of MIR143 candidate targets. (A) Sequence alignment of human hsa-miR-143-3p and pig MIR143. (B) GO pathway analysis of hsa-miR-143-3p experimentally validated targets (MIR143 candidate genes). (C) KEGG pathway analysis of hsa-miR-143-3p experimentally validated targets (MIR143 candidate genes). (D) Predicted results from the RNAhybrid website (https://bibiserv.cebitec.uni-bielefeld.de/rnahybrid/) showed the possibility of hsa-miR-143-3p binding to human AKT1 (verified, blue box) and pig MIR143 binding to pig AKT1 (predicted, green box). Further, Western blotting (WB) showed the AKT protein expression when MIR143 was overexpressed in porcine GCs (red box, MNC indicated MIR143 mimic negative control). (E) Predicted results from the RNAhybrid website (https://bibiserv.cebitec.uni-bielefeld.de/rnahybrid/) showed the possibility of hsa-MIR143 binding to human KRAS (verified, blue box), and MIR143 binding to pig KRAS (predicted, green box). Further, WB showed the effect on KRAS protein expression when MIR143 was overexpressed in porcine GCs (red box, MNC indicated MIR143 mimic negative control).