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. 2020 Oct 19;8:565261. doi: 10.3389/fcell.2020.565261

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Copyright © 2020 Zhong, Li, Chen, Diao, He, Zhang, Zhang, Yuan and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of MIR143 on steroidogenesis and cell apoptosis in pGCs. (A) Hormone measurement in the pGC culture medium. MNC was the MIR143 mimic negative control, and siNC was the siMIR143 negative control. (B) Effect of MIR143 in the expression of key genes related to hormone biosynthesis. (C) Protein analysis of HSD17β4, ER1, ER2, CYP11A1, PTGS2, and CYP19A1 with or without hCG stimulation. The protein relative gray value = protein gray value/β-actin gray value. (D) Cell apoptosis analysis and mRNA levels of cell proliferation markers PCNA, and apoptosis biomarkers BCL2, BAX, and Caspase 3. *P < 0.05, **P < 0.01.