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. 2020 Oct 19;11:561294. doi: 10.3389/fimmu.2020.561294

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Copyright © 2020 Kolomansky, Kaye, Ben-Califa, Gorodov, Awida, Sadovnic, Ibrahim, Liron, Hiram-Bab, Oster, Sarid, Perry, Gabet, Mittelman and Neumann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The number of B-cell-derived preosteoclasts declines following anti-CD20 treatment. (A) Total area of osteoclasts (OC area) grown with M-CSF and RANKL in vitro from bone marrow (BM) non-adherent cells isolated from mice treated with either diluent or anti-CD20. Dashed lines represent the mean ± SEM. (B) B-cell-derived preosteoclasts, defined as CD115⁺/β3⁺ Pro-B cells, in the BM of mice described in (A). Isotype control staining is presented on the left. (C) Bar graphs summarizing the data presented in (B). Data are mean ± SEM of values normalized to the diluent group of each individual experiment. n = 20 and 24 mice in the diluent- and anti-CD20 treatment groups, respectively.