Figure 7.

Psychotropic drugs induce acidic compartment formation perturbing lysosomal and autophagic functioning. Effects of psychotropic drugs on intracellular acidic compartments were evaluated by Lysotracker Deep Red staining after 6 and 16 h of treatment. Nuclei were stained using Hoechst 33342. Pictures were acquired by fluorescence microscopy (magnification: 20×). Representative images of cells treated with DMSO, negative control, rapamycin, positive control, ebastine, fluoxetine, fluspirilene, nefazodone, penfluridol, pimozide, and spiperone (A). Graphs showing quantification of red lysotracker staining/blue nuclei staining ratio as fold change relative to negative control (B). Data are expressed as the mean ± SD of a representative experiment out of three independent experiments performed in triplicate. *p < 0.05; **, Student's T-test p < 0.01; ***, Student's T-test p < 0.001; ****p < 0.0001.