Table 1.

Percentage of reads in the mlaA* library mapping to suppressor genes following three successive overnight incubations in spent medium

Gene name	Percentage of total reads, incubation 1	Percentage of total reads, incubation 2	Percentage of total reads, incubation 3
mlaA	48.1	84.1	86.0
pldA	14.7	9.8	10.3
lptC	8.6	0.9	0
dsbA	4.5	0	0
yaiP	1.3	2.7	1.7
acs	0.7	0	0
fadE	0.6	0	0
secA	0.5	0	0
asmA	0.3	0	0
yejM	0.2	0	0

Genomic DNA was extracted from the mlaA* library following overnight incubation, which induces lysis, and transposon junctions were sequenced. Reads were mapped to the E. coli MC4100 genome and open reading frames were quantified to identify gene disruptions that were enriched after each incubation and hence were potential suppressors. The two known strongest suppressors of mlaA*, mlaA and pldA, quickly predominated in the culture.