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. 2020 Oct 14;117(43):26895–26906. doi: 10.1073/pnas.2011667117

Fig. 2.

Fig. 2.

GIV depletion increases the magnitude and sensitivity of cytokine responses to LPS. (A and B) Bar graphs displaying cytokine transcript levels (qPCR) in GIV-depleted RAW 264.7 macrophages (A) or GIV KO peritoneal macrophages (B) stimulated with LPS (100 ng/mL, 6 h) compared to controls. (C and D) Bar graphs showing levels of secreted proinflammatory cytokines (ELISA) in GIV-depleted RAW 264.7 (C) or GIV KO peritoneal (D) macrophages stimulated with LPS. (E) Line graphs comparing sensitivity of cytokine transcript response to increasing doses of LPS (6 h stimulation) in GIV-depleted RAW 264.7 macrophages compared to controls. All qPCR and ELISA results are from three independent experiments and displayed as mean ± SEM. Student’s t test was used for two-parameter statistical analysis (A and B), and two-way ANOVA using Sidak’s multiple comparisons test was used for multiparameter statistical analysis (C–E). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001; ns, not significant.