Figure 6.

Venn diagram showing 51 overlapping significant pathways of up-regulated and down-regulated innatomic genes in proinflammatory cytokine KO microarray datasets. The significant pathways from down-regulated innate immune genes have the same expression pattern to proinflammatory cytokines. The data showed in 51 common significant pathways, there were about 22 cellular immune response related pathways (account for 43.14%). The data suggested that the up-regulated innate immune genes have the same immune function to the down-regulated innate immune genes by proinflammatory cytokines KO. Additionally, the suppression of PD-1, PD-L1 cancer immunotherapy pathway, Fcγ Receptor-mediated Phagocytosis in Macrophages and Monocytes, Th2 Pathway, Systemic Lupus Erythematosus In T Cell Signaling Pathway, CD27 Signaling in Lymphocytes, PFKFB4 Signaling Pathway and Inflammasome pathway were unique cellular immune response pathways of the up-regulated innate immunity genes after cytokines KO. Activation of IRF by Cytosolic Pattern Recognition Receptors, Th17 Activation Pathway, MIF-mediated Glucocorticoid Regulation, IL-7 Signaling Pathway were unique cellular immune response pathways of the down-regulated innate immunity genes after cytokines KO. The detailed IPA results were showed in Table S7 , S8 . The cellular immune response pathways were marked in bold.