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. 2020 Oct 14;117(43):26804–26811. doi: 10.1073/pnas.2009943117

Fig. 3.

Fig. 3.

Biotin-CoA pull-down assays detect in vivo protein S-CoAlation. (A) P47 cells were treated with biotin or biotin-CoA for 6 h. Cell lysates were prepared and examined by Western blotting for the expression of the indicated proteins (input). For Avidin IP, the same lysates were captured using NeutrAvidin resins, separated by SDS/PAGE, and probed for the proteins indicated. (B) Protein lysates were prepared from P47 cells pretreated with CoA for 1 h followed by biotin or biotin-CoA for 6 h, and examined by Western blotting for the expression of the indicated proteins (input). The same lysates were captured using NeutrAvidin resins, separated by SDS/PAGE, and probed for the proteins indicated. (C) The same lysates as in A were treated with BMH and examined for the migration/structure of the indicated proteins.