Figure 6.

Transplantation of CB-HSPCs produces a longer duration of multi-lineage engraftment than BM-HSPCs. Analyses were performed to evaluate the duration of the multi-lineage expansion produced by the initial HSPC transplantation as well as the ability of engrafted HSPC to re-populate a naïve host. (A) Blood draws were performed every 3 weeks on NBSGW given 2.5E⁶ CB-HSPCs until week 12 after which they were performed every 12 weeks until 36 weeks. (B) Bar graph showing the total number of human cells in the bone marrow collected from one leg (femur + tibia) as determined by flow cytometry in mice 12 or 36 weeks post-transplant. The lineage composition of the human cells is shown as a pie graph. (C) Bone marrow collected from four mice initially transplanted with 2.5E⁶ CB-HSPCs was combined and subjected to density gradient centrifugation, then 3E⁷ mononuclear cells was transplanted into each of four naïve NBSGW mice. The number of transplanted HSPCs was approximately 2.5E⁶/mouse. The plot shows total number of human cells in the bone marrow collected from one leg (femur + tibia) at 12 weeks after primary vs. secondary transplant. Pie chart shows lineage composition at 12 weeks after secondary transplant. (D–F) Results from similar analysis using BM-HSPCs. Symbols show results from individual mice, and lines or bars show the geometric means. *p < 0.05; **p < 0.01.