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. 2020 Oct 28;12:10651–10667. doi: 10.2147/CMAR.S259077

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© 2020 Zhuang et al.

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MiR-21 targets and inversely regulates SPRY1. The lentiviruses containing miR-21 agomir, miR-21 antagomir or their corresponding NCs were infected into the cells. (A) The specific binding site between SPRY1 and miR-21 predicted by online analysis. (B) The binding of miR-21 to SPRY1 3ʹUTR confirmed by dual luciferase reporter assay. (C) The expression pattern of miR-21 in the human normal esophageal epithelial cell line (Het-1A) and human ESCC cell lines (EC9706, Eca109, KYSE-150, TE-1, and TE-5) determined using RT-qPCR. (D) The mRNA expression pattern of SPRY1 determined using RT-qPCR. (E) The protein expression pattern of SPRY1 determined using immunoblotting. *p < 0.05 vs cells treated with agomir-NC. Unpaired t-test was adopted for data comparisons between two groups. Comparisons among multiple groups were conducted by one-way ANOVA, followed by a Tukey’s post hoc test. The experiment was conducted three times independently.