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. 2020 Oct 28;12:10651–10667. doi: 10.2147/CMAR.S259077

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© 2020 Zhuang et al.

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KYSE-150 cells secrete exosomes. (A) The morphological observation of exosomes under TEM (scale bar = 200 nm). (B) Nanoparticle tracking analysis of exosomes. (C) The protein expression pattern of exosome markers CD9, CD63, TSG101, and GRP94 as detected by Western blot analysis (GRP94: 92 kDa, TSG101: 44 kDa, CD9: 25 kDa, CD63: 26 kDa). (D) Expression pattern of miR-21 in the exosomes of ESCC cells as determined using RT-qPCR. *p < 0.05 vs Het-1A cells.