Table 2.

Primer Sequences for RT-qPCR

Gene of Interest	Primer Sequence (5ʹ-3ʹ)
miR-21	F: GCCCGCTAGCTTATCAGACTGATG	R: GTGCAGGGTCCGAGGT
SPRY1	F: GCAGTGGCAGTTCGTTAGTTG	R: CAGTAGGCTGAATCTCTCTCTCA
VEGF	F: CAAACGCTGACATGTACGGTCTA	R: CCAACTGCCAATACCAGTGGAT
U6	F: CGCTTCGGCAGCACATATACTA	R: CGCTTCACGAATTTGCGTGTCA
GAPDH	F: TCACCCACACTGTGCCCATCTACGA	R: CAGCGGAACCGCTCATTGCCAATGG

Abbreviations: RT-qPCR, reverse transcription quantitative polymerase chain reaction; miR-21, microRNA-21; SPRY1, Sprouty RTK signaling antagonist 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; U6, small nuclear RNA; F, forward; R, reverse.