TABLE 1.
Generated reads and mapping statistics of Methyl-RAD Seq sequence data from Staphylococcus aureus induced and healthy udder tissues of Chinese Holstein cows.
Samples | Raw reads | Clean reads | Error rate | Enzyme reads | Q20 | Q30 | Multi mapped reads | Multi-mapping rate | Unique mapped reads | Unique mapping rate |
A1 | 13479949 | 13467408 | 0.99907 | 12910973 | 99.87% | 99.67% | 9053897 | 70.13% | 3857076 | 29.87% |
B1 | 13724935 | 13711869 | 0.999048 | 13251576 | 99.88% | 99.70% | 9151743 | 69.06% | 4099833 | 30.94% |
C1 | 13411585 | 13398863 | 0.999051 | 12483616 | 99.88% | 99.70% | 8464402 | 67.80% | 4019214 | 32.20% |
A2 | 14104057 | 14090675 | 0.999051 | 13650097 | 99.88% | 99.69% | 9381653 | 68.73% | 4268444 | 31.27% |
B2 | 13165243 | 13152778 | 0.999053 | 12812360 | 99.88% | 99.69% | 8932597 | 69.72% | 3879763 | 30.28% |
C2 | 13239916 | 13226976 | 0.999023 | 12399152 | 99.88% | 99.69% | 8394779 | 67.70% | 4004373 | 32.30% |
A1, B1, and C1 represents healthy udder tissues of Chinese Holstein cows (CL group); A2, B2, and C2 represents S. aureus mastitis infected udder tissues of Chinese Holstein cows (SA group); Error Rate is the ratio of clean reads to raw reads; Enzyme Reads represent clean reads containing the expected restriction site and that passed quality control. The multi/unique-mapping rate is based on enzyme reads.