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. 2020 Oct 28;12:10559–10572. doi: 10.2147/CMAR.S272498

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© 2020 Liu et al.

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PTPN4 is a negative regulator of the STAT3 in OSCC cells. (A) protein expression and phosphorylation of STAT3 in cells transfected with si-PTPN4 determined by Western blot analysis (*p < 0.05 according to two-way ANOVA); (B) protein expression and phosphorylation of STAT3 in HIOEC, SCC-4 and CAL-27 cells determined by Western blot analysis; (C) protein expression and phosphorylation of STAT3 in SCC-4 and CAL-27 cells after further STAT3-IN-7 treatment determined by Western blot analysis (*p < 0.05 according to two-way ANOVA); (D) apoptosis rate in SCC-4 and CAL-27 cells determined by flow cytometry (*p < 0.05 according to two-way ANOVA). Repetition = 3.