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Primer Sequences for RT-qPCR
| Gene | Primer Sequence (5ʹ-3ʹ) |
|---|---|
| miR-15b-5p | F: TAGCAGCACATCATGGTTTACA |
| R: TGCGTGTCGTGGAGTC | |
| PTPN4 | F: ATCTCCACCGGGAACTCCTA |
| R: CGCTTGGGGAAGTATGAACCA | |
| U6 | F: CTCGCTTCGGCAGCACA |
| R: AACGCTTCACGAATTTGCGT | |
| GAPDH | F: CTAAGGCCAACCGTGAAAAG |
| R: ACCAGAGGCATACAGGGACA |
Abbreviations: RT-qPCR, reverse transcription quantitative polymerase chain reaction; miR, microRNA; PTPN4, phosphatase non-receptor type 4; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F, forward; R, reverse.
