Table 2.
Effects of SIF incubation on antibody function evaluated by Hemagglutination Inhibition Assay. The anti-ETEC mAbs 3 and 4 were incubated in SIF, and samples from multiple time points evaluated for their ability to inhibit hemagglutination. Each assay was performed twice and the averaged result is reported as the minimal concentration at which inhibition was observed (IC100 in μg/mL). The highest concentration tested for inhibition was 1.25 μg/mL.
|
mAb3 |
mAb4 |
|||
|---|---|---|---|---|
| SIgA1 | SIgA2 | SIgA1 | SIgA2 | |
| SIF control | >1.25 | >1.25 | >1.25 | >1.25 |
| No SIF | 0.64 | 0.94 | 0.94 | 0.94 |
| T = 5 | 0.10 | 0.16 | 0.16 | >1.25 |
| T = 30 | 0.39 | >1.25 | 0.31 | >1.25 |
| T = 60 | >1.25 | >1.25 | 0.63 | >1.25 |
| T = 120 | >1.25 | >1.25 | 1.25 | >1.25 |