Figure EV2. BALOs express markers of differentiated airways and alveoli.

• A, B
  Representative scheme and confocal images of the BALO alveolar‐like (A) and airway‐like (B) structures. (A) Representative image of AEC II SFTPC staining (white asterisks) and AEC I endogenous PDPN expression in day 28 BALO isolated from the lungs of Pdpn ^GFP reporter mice. Scale bars represent 25 μm. Dotted lines indicate the alveoli. (B) Representative fluorescence images of BALO's airway‐like structures stained for β‐IV tubulin⁺ ciliated cells (red arrowheads) and SCGB1A1⁺ club cells. Scale bars represent 25 μm (left and middle) and 5 μm (right).