Skip to main content
. 2020 Sep 16;39(21):e105139. doi: 10.15252/embj.2020105139

Figure 1. Deficiency of MARCH2 strengthens antimicrobial immunity in vivo .

Figure 1

  • A, B
    MARCH2+/+ (n = 8) or MARCH2−/− (n = 8) mice were intravenously infected with VSV‐GFP (2 × 108 PFU/mouse), and serum samples were collected at 12 h post‐infection (hpi). (A) Virus titer was analyzed in a plaque assay. (B) Secretion of IFN‐β, IL‐6, IL‐12, and TNF‐α was measured in specific ELISAs.
  • C
    MARCH2+/+ (n = 6) or MARCH2−/− (n = 6) mice were intravenously injected with poly(I:C) (200 μg/mouse). Serum samples were collected at 2 hpi, and secretion of IFN‐β, IL‐6, IL‐12, and TNF‐α was measured in specific ELISAs.
  • D–J
    MARCH2+/+ (n = 8) or MARCH2−/− (n = 8) mice were intraperitoneally challenged with 1 × 106 CFU of Listeria monocytogenes. (D) The percentage of surviving mice (D, log‐rank test, **P < 0.01) and body weight changes for each group (E) are shown. (F, G) At 3 dpc, the bacterial load in the spleen (F) and liver (G) was examined. (H–J) Amount of IL‐6, IL‐12, CCL5, and CXCL10 in serum (H), spleen (I), and liver (J) of mice in each group at 24 hpc.
Data information: *P < 0.05, **P < 0.01 (two‐tailed Student's t‐test). Data are expressed as the mean ± SEM.Source data are available online for this figure.