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. 2020 Sep 16;39(21):e105139. doi: 10.15252/embj.2020105139

Figure 2. Deficiency of MARCH2 induces susceptibility to LPS‐induced septic shock.

Figure 2

  • A, B
    MARCH2+/+ (n = 8) or MARCH2−/− (n = 8) mice were challenged intraperitoneally with LPS (24 mg/kg). (A, B) Percentage of surviving mice (A, log‐rank test, **P < 0.01) and body weight changes (B) in each group.
  • C
    MARCH2+/+ (n = 4) or MARCH2−/− (n = 4) mice were challenged intraperitoneally with LPS (24 mg/kg). Representing slides of H&E staining of spleen sections from each group. Scale bar, 6 mm.
  • D
    MARCH2+/+ (n = 6) or MARCH2−/− (n = 6) mice were challenged intraperitoneally with LPS (24 mg/kg). Levels of IL‐6, TNF‐α, CXCL‐10, and CCL‐5 in serum from mice in each group were measured at 12 hpc by ELISA.
  • E, F
    MARCH2+/+ (n = 5) or MARCH2−/− (n = 5) mice were challenged intraperitoneally with LPS (24 mg/kg). cDNA was prepared from total RNA extracted from spleen and liver of mice. Expression of mRNA encoding IL‐6, TNF‐α, CXCL10, and IL‐1β in spleen (E) and liver (F) from mice in each group was examined at 6 hpc by qPCR.
Data information: *P < 0.05, **P < 0.01, ***P < 0.001 (two‐tailed Student's t‐test). Data are expressed as the mean ± SEM.Source data are available online for this figure.