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. 2020 Sep 16;39(21):e105139. doi: 10.15252/embj.2020105139

Figure EV5. MARCH2 leads to NEMO degradation, but not its dissociation from IKK complex via interaction with CC1 domain.

Figure EV5

  • A
    HEK293T cells were transfected with HA‐tagged NEMO‐WT or NEMO‐∆CC1 in the absence or presence of Strep‐tagged MARCH2. Whole‐cell lysates were subjected to immunoblotting with anti‐HA, anti‐Strep, or anti‐β‐actin antibody.
  • B
    Schematic presentation of NEMO domain, interacting with IKK complex (IKKα, IKKβ) or MARCH2.
  • C, D
    HEK293T cells transfected with HA‐tagged (C) IKKα or (D) IKKβ together with indicated plasmids were treated with MG132 (10 μM) for 6 h before harvest. Whole‐cell lysates were subjected to immunoprecipitation with an anti‐HA antibody, followed by immunoblotting with an anti‐HA, anti‐Strep, or anti‐GST antibody. Whole‐cell lysate was subjected to above antibody together with anti‐β‐actin.
Data information: Data are representative of at least two independent experiments, each with similar results. Source data are available online for this figure.