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. 2020 Sep 15;39(21):e104472. doi: 10.15252/embj.2020104472

Figure EV1. Expression of dnLEF reduces canonical Wnt signaling activity in adult‐born granule neurons.

Figure EV1

  1. Experimental scheme of retroviral injection paradigm. Adult BATGAL mice were stereotactically injected with either the CAG‐dnLEF‐IRES-GFP (dnLEF) or CAG‐GFP (control) MMLV and were sacrificed 17 dpi.
  2. Representative images of β‐galactosidase reporter expression (red) in transduced neurons (green). Arrows and arrowheads point to transduced neurons with and without reporter signal, respectively. Scale bar = 10 μm. Insets show magnifications of transduced cells. Scale bar = 5 μm.
  3. Measurements of the corrected total cell fluorescence of the β‐galactosidase signal in control and dnLEF neurons show reduced canonical Wnt signaling activity in neurons expressing dnLEF (P < 0.0001; control: n = 47 cells from three animals, dnLEF: n = 35 cells from three animals).
Data information: Data represented as mean ± SEM, significance was determined using two‐tailed Mann–Whitney U‐test, and significance levels are displayed in GP style (****P < 0.0001).