Figure 4.

Flow cytometry analysis in lung tissue single-cell suspension. The rats were randomly divided into five groups. Briefly, the rats in the SM group received sham operation, while the left lung hilum was subjected to 1 h of ischemia followed by reperfusion up to 3 h in the IR group. Intraperitoneal Nec-1 (1 mg/kg) and z-VAD-fmk (3 mg/kg) were administrated before lung ischemia in NI and ZI groups, respectively, and combined administration of Nec-1 and z-VAD-fmk was given in the NZ group. After 3 h of reperfusion, single-cell suspension of the lung tissue was prepared, and cell death was assessed using flow cytometry. Figures 4(a) and 4(b) are representative photomicrographs of different groups. Data were presented as mean ± SD, n = 8 in each group. ^aP < 0.05 compared with the SM group, ^bP < 0.05 compared with the IR group, ^cP < 0.05 compared with the NI group, ^dP < 0.05 compared with the ZI group.