Skip to main content
. 2020 Oct 23;2020:2048210. doi: 10.1155/2020/2048210

Figure 5.

Figure 5

SMARCB1 mediates the ubiquitination of NR4A3. (a) Relative mRNA expression of NR4A3 in HUVEC (left panel) and RAOEC (right panel) while SMARCB1 being knockdown. (b) Representative blot of protein expression of NR4A3 in HUVEC (left panel) and RAOEC (right panel) while SMARCB1 being knockdown. (c) Quantification of NR4A3 protein expression was determined by the grayscale of blots from three independent experiments and normalized to GAPDH. (d) Determination of ubiquitination of NR4A3 in HUVEC (left panel) and RAOEC (right panel) while SMARCB1 being knockdown by the antiubiquitin IP assay followed by western blot. After SMARCB1 being knockdown for 24 hours, 100 nM MG132 was applied for an additional 24-hour incubation followed by sample collection for the IP assay. Statistical analysis of (a) and (c) was performed using one-way ANOVA, followed by Tukey's multiple comparison tests; n.s.: no significance; ∗∗p < 0.01 and ∗∗∗p < 0.001. RNAi: RNA interference; Ctrl: normal cultured cells; siNC: cells subjected to treatment with negative scrambled small interfering RNA; siSMARCB1: cells subjected to treatment with small interfering RNA against SMARCB1.