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Effect of lactate on H3 acetylation during cisplatin treatment. Wild-type BY4741 cells were grown to early exponential phase in SC-glucose medium (pH 5.5). Cells were washed and exposed to 600 μg/mL cisplatin in SC medium containing glucose or lactate for up to 180 minutes. H3 acetylation levels were assessed by Western blot. Pgk1p levels were used as a loading control. A representative image from 3 independent experiments is shown.
