Figure 3. G3 Tert ^−/− mice display defects in the NAD metabolism.

• A, B
  Intracellular NAD levels and immunoblots of the expression of NAD consuming‐related proteins and their activities in brain tissues from 6‐month‐old G1 and G3 Tert ^−/− female mice. The CD38 antibody was validated using the CD38 ^−/− mouse brain tissues.
• C
  Quantification of immunoblots from the indicated proteins in (B).
• D
  NADase activity in G1 and G3 Tert ^−/− mouse brain lysate was determined at various time points during the reaction. NADase activity was not detectable in the extracts treated with the CD38 inhibitor, 78c, at a concentration of 50 nM.

Data information: All values are presented as mean ± SD of G1 Tert ^−/− mouse brain tissues vs G3 Tert ^−/− mouse brain tissues. n = 4 in each group. P values on the basis of Student's t‐test.