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. 2020 Sep 18;39(21):e106003. doi: 10.15252/embj.2020106003

Figure EV2. CRISPR‐Cas9 editing of the RAB 3′UTR in vitro and in vivo does not generate off‐target mutations.

Figure EV2

  1. Chromatogram confirming the excision of the LE within RAB13 3′UTR in HUVECs.
  2. List of predicted CRISPR‐Cas9 off‐target genes and RNAseq mismatch detection in CRISPR‐Cas9-derived HUVEC clones (n = 1 each genotype).
  3. Chromatogram confirming the CRISPR‐Cas9-mediated excision of 482‐nt within the rab13 3′UTR in zebrafish embryos.
  4. List of predicted CRISPR‐Cas9 off‐target genes and RNAseq mismatch detection in Tg(kdrl:EGFP) rab13 +/+ and rab13 ∆3′UTR/∆3′UTR embryos (n = 2 each genotype).