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. 2020 Sep 18;39(21):e106003. doi: 10.15252/embj.2020106003

Figure 8. rab13 mRNA polarisation orients blood vessel morphogenesis.

Figure 8

  1. Time‐lapse confocal microscopy of representative Tg(kdrl:EGFP) rab13 +/+ and rab13 ∆3′UTR/∆3′UTR embryos. DLAV: dorsal longitudinal anastomotic vessel; HM: horizontal myoseptum.
  2. Frequency of ISV ectopic branching occurring at the HM (n = 4 experiments; *P < 0.05; one‐way ANOVA with Bonferroni's correction).
  3. Illustration of the role for RAB13 mRNA localisation, local translation and compartmentalisation of RAB13 function in defining the orientation of EC filopodia dynamics, motile EC polarity and blood vessel pathfinding.
Data information: T 0 = 25 hpf; arrowheads indicate extra branches emerging from the main ISVs at the HM position; scale bars = 50 μm (A). +/+, +/∆ and ∆/∆ represent Tg(kdrl:EGFP) rab13 +/+, rab13 +/∆3′UTR and rab13 ∆3′UTR/∆3′UTR embryos, respectively (B). Bar chart is presented as means ± s.d.