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. 2020 Sep 16;39(21):e99520. doi: 10.15252/embj.201899520

Figure 1. Quantitative analysis of RT shifts reveals cooperation between two combinations of cis‐regulatory elements.

Figure 1

Distribution of RT shifts calculated with the –ΔL + ΔE method described in Appendix Fig S1. Different transgenes in a mid‐late‐replicating region are compared: the IL2R reporter gene under the control of the β A ‐globin promoter (β A pro) containing an inactive (i, N = 10) or an active origin (ii, N = 8) flanked by two copies of FIV (2xFIV), the blasticidin resistance gene (BsR) under the control of the β‐actin promoter (β‐act pro) (iii, N = 7), or a combination of these two transgenes (iv, N = 8). Blue and black triangles represent reactive loxP sites and recombined inactive loxP sites, respectively. Rectangle edges correspond to the 0.25 and 0.75 quartiles, the thick black lines represent the median, the white triangles represent the mean, and the whiskers extend to the smallest and largest –ΔL + ΔE values. Statistical analysis was performed with Wilcoxon nonparametric two‐tailed tests (ns, not significant; **P < 0.01; ***P < 0.001).