Figure 2. One autonomous replicon inserted in the mid‐late region perturbs the RT over a 250 kb region through cooperation with an endogenous strong origin.

1. UCSC genome browser visualization of the mid‐late insertion site of chromosome 1 (genomic positions: chr1:71,000,000–74,100,000 bp, 3.1 Mb; galGal5). RT‐weighted average (WA) values for the wt and the β ^A ‐globin + β‐actin cell lines are shown. Early‐replicated regions (E) are represented in orange and late‐replicating regions (L) in blue. Annotated genes (Ref Seq genes) are represented below. The mid‐late insertion site is indicated with a red arrow and dotted line.
2. UCSC genome browser visualization of the mid‐late insertion site of chromosome 1 (genomic positions: chr1:72,450,000–72,650,000 bp; 200 kb; galGal5). Tracks of nascent strands (NS) enrichments in the four S‐phase fractions were represented separately (S1–S4) for the wt and the 2 × (β ^A ‐globin + β‐actin) cell lines. NS‐enriched and depleted regions for each fraction are represented in purple and blue, respectively. Single reads form SNS aligned and track of replication origins (Ori peaks) determined in (Massip et al, 2019) are reported in between. The three mid‐late insertion sites 1, 2, and 3 are indicated with red arrows. Annotated genes and CpG Islands are shown below. Initiation zones (IZ) and termination zones (TZ) are reported.