Figure EV2. One advanced replicon inserted at site 1 or at site 3 directs a shift to earlier replication independently of the presence of a GFP reporter construct inserted at site 2.

RT profiles of each chromosomal allele are determined after targeted transgene integration using the allele‐specific analysis of RT method by real‐time PCR quantification described in Appendix Fig S1. Differences in –ΔL + ΔE values calculated at the target site following transgene integration are indicated.

• A
  Analysis of one clonal cell line containing one GFP reporter construct composed of the GFP reporter gene under the control of the β ^A ‐globin promoter (β ^A pro) and linked to a 1.6 kb fragment of human chromosome 7 (h.K7) inserted at site 2.
• B, C
  Analysis of clonal cell lines containing one β ^A ‐globin + β‐actin construct described in Fig 1 inserted at site 1 and one GFP reporter construct inserted at site 2 on the same chromosome (B) or on the other chromosome (C).
• D, E
  Analysis of clonal cell lines containing one β ^A ‐globin+β‐actin construct inserted at site 3 and one GFP reporter construct inserted at site 2 on the same chromosome (D) or on the other chromosome (E).

Data information: Blue and black triangles represent reactive loxP sites and recombined inactive loxP sites, respectively. Black vertical bars represent insertion sites. Error bars correspond to the standard deviation for qPCR duplicates.