Figure EV3. Two β ^A ‐globin + β‐actin constructs as well as two β‐actin constructs inserted at sites 1 and 3 form an early‐replicated domain.

• A–C
  RT profiles of each chromosomal allele are determined after targeted transgene integration using the allele‐specific analysis of RT method by real‐time PCR quantification described in Appendix Fig S1. Differences in −ΔL + ΔE values calculated at the target site following transgene integration are indicated. Error bars correspond to the standard deviation for qPCR duplicates. (A, B) Analysis of two 1 + 2 + 3 (A) and three 1 + 3 (B) clonal cell lines described in Fig 3A. (C) Analysis of five clonal cell lines containing two β‐actin constructs inserted at sites 1 and 3 on the same chromosome described in Fig 3B. Blue and black triangles represent reactive loxP sites and recombined inactive loxP sites, respectively. Black vertical bars represent insertion sites. Error bars correspond to the standard deviation for qPCR duplicates.