Figure EV5. Two β ^A ‐globin constructs inserted at sites 1 and 3 are not sufficient to form an early‐replicated domain.

RT profiles of each chromosomal allele are determined after targeted transgene integration using the allele‐specific analysis of RT method by real‐time PCR quantification described in Appendix Fig S1. Differences in –ΔL + ΔE values calculated at the target site following transgene integration are indicated. Error bars correspond to the standard deviation for qPCR duplicates. Analysis of six β ^A ‐globin (1 + 3) clonal cell lines described in Fig 4B.