Fig. 2.

Expression of thermogenic and nutrient handling genes in iBAT of WT and Ucp1^−/− mice. BAT samples from male WT and Ucp1^−/− mice acclimated to 22°C or 30°C were harvested for mRNA and protein analyses (n = 5–6). A: mRNA expression of indicated genes in iBAT, values are shown relative to WT 22°C. B: iBAT Western blot analysis using specific antibodies to indicated proteins. C: Quantification of Western blots, values were normalized to the housekeeper g-TUB and shown relative to WT 22°C. *P < 0.05 by two-way ANOVA. Only significant differences between genotypes in the same interventional group are depicted. HMW, high-molecular-weight; LMW, low-molecular-weight.