Fig. 3.

UCP1 deficiency alters energy disposal in thermogenic adipose tissues of cold-acclimated mice. Female WT and Ucp1^−/− mice were gradually acclimated to 6°C and gene expression as well as nutrient uptake were measured. For this purpose, mice were kept for 1 week at 18°C and then acclimated to 6°C for 3 weeks before being subjected to an OGFT (n = 8–10). Specific (A) and total (B) ¹⁴C-triolein uptake into iBAT. Specific (C) and total (D) ³H-DOG uptake into iBAT. Expression of mRNA (E) shown relative to WT and protein (F) by Western blot of iBAT samples. G: Quantification of Western blots, values were normalized to g-TUB and shown relative to WT. Total iWAT uptake of ¹⁴C-triolein (H) and ³H-DOG (I). J: iWAT gene expression normalized to Tbp, values are shown relative to WT. *P < 0.05 by Student’s t-test.