TABLE 1.
Summary of linear ranges and LODs and LLOQs for BAs in this assay
| Analyte | LOD (ng/ml) | Linear range (ng/ml) | r2 |
| LCA | 0.5 | 0.5–2,500 | 0.991 |
| CDCA | 0.5 | 0.5–2,500 | 0.991 |
| DCA | 0.1 | 0.5–2,500 | 0.995 |
| UDCA | 0.5 | 0.5–2,500 | 0.997 |
| CA | 0.1 | 0.5–2,500 | 0.990 |
| ACA | 0.5 | 1–2,500 | 0.991 |
| GLCA | 0.05 | 0.5–2,500 | 0.998 |
| GDCA | 0.05 | 0.5–2,500 | 0.999 |
| GUDCA | 0.05 | 0.5–2,500 | 0.999 |
| GCDCA | 0.05 | 0.5–2,500 | 0.997 |
| GCA | 0.1 | 0.5–2,500 | 0.999 |
| TLCA | 0.05 | 0.1–2,500 | 0.997 |
| TDCA | 1 | 5–2,500 | 0.995 |
| TCDCA | 0.1 | 1–2,500 | 0.997 |
| TCA | 0.5 | 1–2,500 | 0.999 |
| IALCA | 5 | 5–2,500 | 0.992 |
| 3-oxo-chol-4-enic acid | 10 | 10–2,500 | 0.990 |
| 3-oxo-CA | 0.5 | 5–2,500 | 0.990 |
| 7α-hydroxy-3-oxo-chol-4-en-24-oic acid | 0.5 | 1–2,500 | 0.996 |
These values were determined using authentic standards dissolved in mobile phase. LOD is defined as analyte peak area having S/N >3. LLOQ is represented as the lower end of the linear range and is defined as analyte peak area having S/N >10 and deviation from the nominal value less than ±20%. The r2 value reflects fit to linear regression with 1/x weighting.