Table 1:
Statistical Analysis
| Fig. # | Analysis type and animal numbers |
|---|---|
| 1A-D | At 55 lux: eYFP n=8, ChR2 n=11; At 2.7 × 104 lux: eYFP n=12, ChR2 n=18 |
| 1A | A three-way ANOVA revealed no statistical difference between light intensity: F (1,45) = 0.524, p = 0.47. Therefore, a two-way ANOVA was done to analyze treatment and time. Two-way ANOVA: At 55 lux: Interaction: F(5,85)=0.6434, p=0.67; Treatment: F(1,17)=14.41, p=0.001; Time: F(5,85)=0.1571, p=0.98. Holm-Sidak’s multiple comparisons test: *p<0.05, ** p<0.01. At 2.7 × 104 lux: Interaction: F(5,140)=0.318, p=0.90; Treatment: F(1,28)=11.67, p=0.002; Time: F(5,140)=0.842, p=0.52. Holm-Sidak’s multiple comparisons test: #p<0.05 |
| 1B | Unpaired two-tailed t-test: At 55 lux: t=3.80, df=17, p=0.001; At 2.7 × 104 lux: t=3.42, df=28, p=0.002 |
| 1C | Unpaired two-tailed t-test: At 55 lux: t=0.24, df=17, p=0.81; At 2.7 × 104 lux: t=0.25, df=28, p=0.81 |
| 1D | Unpaired two-tailed t-test: At 55 lux: t=2.17, df=17, *p=0.044; At 2.7 × 104 lux: t=2.76, df=28, p=0.009 |
| 1E | Unpaired two-tailed t-test: t=0.97, df=15, p=0.35; eYFP n=8, ChR2 n=9 |
| 3A-D | Veh n=26, CGRP 1.0 μg n=9, 0.5 μg n=13, 0.1 μg n=13 |
| 3A | Two-way ANOVA: Interaction: F(15,285)=2.147, p=0.0083; Treatment: F(3,57)=4.842, p=0.0045; Time: F(5,285)=3.381, p=0.006. Holm-Sidak’s multiple comparisons test: ***p<0.001, **p<0.01, *p<0.05 CGRP 1.0 μg vs Veh; ##p < 0.01, #p<0.5 CGRP 0.5 μg vs Veh; ^p<0.05 CGRP 0.1 μg vs Veh |
| 3B | One-way ANOVA: F(3,57)=6.045, p=0.001. Holm-Sidak’s multiple comparisons test: ***p<0.001, #p<0.05, ^p<0.05 |
| 3C | One-way ANOVA: F(3,57)=3.311, p=0.026. Holm-Sidak’s multiple comparisons test: #p<0.05 |
| 3D | One-way ANOVA: F(3,57)=3.451, p=0.022. Holm-Sidak’s multiple comparisons test: **p<0.01 |
| 3E | Unpaired two-tailed t-test: t=1.13, df=18, p = 0.27,; Veh n=9, CGRP n=11 |
| 5A-D | eYFP n=9, ChR2 n=10 |
| 5A | Two-way ANOVA: Interaction: F(5,85)=3.06, p=0.014; Treatment: F(1,17)=5.608, p=0.030; Time: F(5,85)=10.06, p<0.001. Holm-Sidak’s multiple comparisons test: *p<0.05 |
| 5B | Unpaired two-tailed t-test: t=2.13, df=17, p=0.047 |
| 5C | Unpaired two-tailed t-test: t=1.171, df=17, p=0.26 |
| 5D | Unpaired two-tailed t-test: t=4.004, df=17 p<0.001 |
| 5E | Unpaired two-tailed t-test: t=7.025, df=14, p<0.001; eYFP n=6, ChR2 n=10 |
| 6A-F | Veh n=14, CGRP n=22 |
| 6A | Two-way ANOVA: Interaction: F(5,55)=0.109, p=0..99; Treatment: F(1,11)=0.136, p=0.72; Time: F(5,55)=1.664, p=0.16. |
| 6B | Unpaired two-tailed t-test: t=0.952, df=34, p=0.35 |
| 6C | Unpaired two-tailed t-test: t=0.983, df=34, p=0.33 |
| 6D | Unpaired two-tailed t-test: t=1.25, df=34, p=0.22 |
| 6E | Unpaired two-tailed t-test: t=0.23, df=16, p=0.83; Veh n=9, CGRP n=9 |
| 7A-D | eYFP n=11, ChR2 n=11 |
| 7A | Two-way ANOVA: Interaction: F(5,100)=3.03, p=0.014; Time: F(5,100)=0.471, p=0.80; Treatment: F(1,20)=0.127, p=0.73 |
| 7B | Unpaired two-tailed t-test: t=0.356, df=20, p=0.73 |
| 7C | Unpaired two-tailed t-test: t=0.293, df=20, p=0.77 |
| 7D | Unpaired two-tailed t-test: t=1.340, df=20, p=0.20 |
| 7E | Unpaired two-tailed t-test: t=1.10, df=19, p=0.29, eYFP n=10, ChR2 n=11 |
| 8A-D | Veh n=12, CGRP n=11 |
| 8A | Two-way ANOVA: Interaction: F(5,105)=1.039, p=0.40; Time: F(5,105)=1.384, p=0.24; Treatment: F(1,21)=0.128, p=0.72 |
| 8B | Unpaired two-tailed t-test: t=0.358, df=21, p=0.72 |
| 8C | Unpaired two-tailed t-test: t=2.288, df=21, p=0.033 |
| 8D | Unpaired two-tailed t-test: t=0.901, df=21, p=0.38 |
| 8E | Unpaired two-tailed t-test:, t=0.306, df=19, p=0.76, eYFP n=10, ChR2 n=11 |