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. 2020 Oct 27;12:10639–10649. doi: 10.2147/CMAR.S268216

Figure 2.

Figure 2

CYLD overexpression inhibits NPC cell proliferation. (A) CYLD protein levels measured by Western blot in two immortalized normal human nasopharyngeal epithelial cell lines and six NPC cell lines. β-actin (ACTB) was used as a loading control. (B) CNE2 and HK1-EBV cells stably overexpressed CYLD, whereas HONE1 and 5–8F cells were transfected with scrambled siRNA or CYLD-specific siRNA, and CYLD levels were detected by Western blot. (C) CCK-8 and (D, E) colony formation assays were used to evaluate cell growth and proliferation. Data represent the mean ± SEM (n =3). ***P < 0.001.