Figure 3.

HFRT increased pericyte-coverage in tumor vessels more than CRT. (a) and (b) Fluorescence microscopy images of CD34-positive endothelial cells and α-SMA-positive pericytes (red and green, respectively, 400×) of each group on day 7 and day 14. (c) and (d) The ratio of a-SMA/CD34 was measured by calculating the number of CD34-positive spots overlapping α-SMA-positive spots to the total number of CD34-positive spots in five randomly selected sets of 40 high-magnification (400×) fields per slide, of five slides per tumor (*p < 0.05, one-way analysis of variance test).

α-SMA, anti-α smooth muscle actin; CRT, conventional radiation therapy; d, day; HFRT, hypo-fractionation radiotherapy.