Figure 4.

Microscopic pictures of rats’ testes immunostained against inflammatory NF-κB (A–D) and statistical analysis of IHC intensity scores of NF-κB expression (E). Testicular sections showed a weak positive brown expression for NF-κB expression in the NC and GIN C groups (A and B), a strong positive brown staining for NF-κB expressions in the MTX group in the germ cells (C), a moderate positive brown staining for NF-κB expressions in the GIN+MTX treated group (D). IHC counterstained with Mayer’s hematoxylin. X400, bar = 50 µm. ***P < .05 versus NC, ^#P < .05 versus MTX.