Figure 4.

Platelet dense granule secretion is enhanced in JUUL exposed mice. Platelets from clean air- or JUUL e-cigarette-exposed mice were incubated with 12.5 µL of luciferase luciferin, before activation with 1 μM ADP (A) or 0.1 U/mL thrombin (B). Dense granule/ATP release was assessed by a lumi-aggregometer and detected as luminescence. Inset shows quantification of data; **P < .01 and ***P < .0001.These experiments were repeated 3 times, using blood that was pooled from 5 to 8 mice each time.