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Michaelis-Menten plots of pNPA hydrolysis promoted by a) 7 and the mixture (24:76) of 1 and 7; and b) 4 and the mixture (50:50) of 1 and 4. Experimental conditions: 25 mM Tris, pH 8.0, 25 μM total peptide, 0.1 mM Zn2+. 25 μM peptide, 6.25 μM active 2–7 species, the samples were incubated for 24 h prior to measurements.
