Skip to main content
. 2020 Oct 29;76(Pt 11):536–543. doi: 10.1107/S2053230X20013874

Figure 2.

Figure 2

Preparation of SmChiP expressed in E. coli. (a) A representative elution profile of SmChiP eluted from a HiPrep 16/100 Sephacryl S-200 HR column with 10 mM HEPES pH 7.4, 100 mM LiCl, 0.1% LDAO (upper panel). SDS–PAGE showed that a single protein peak with an elution volume of 65–100 ml contained SmChiP as the major component (lower panel). (b) SDS–polyacrylamide gel showing migration of the purified SmChiP after heating at 373 K for 10 min (lane +) and without heat treatment (lane −). Lane Std contains molecular-weight markers (labelled in kDa). (c) Isotopic ionization pattern of SmChiP as determined by electrospray mass spectrometry. The inset shows the peak deconvolution.