Table 3. Kinetic parameters for wild-type and T89S mutant FtFBPaseII.
FtFBPaseII | K m (µM) | V max (µmol min−1 mg−1) | k cat (s−1) | k cat/K m (s−1 mM −1) |
---|---|---|---|---|
Wild type | 17 ± 4 | 0.9 ± 0.02 | 0.55 ± 0.04 | 33 |
T89S | 4 ± 2.1 | 0.2 ± 0.03 | 0.12 ± 0.02 | 31 |
FtFBPaseII | K m (µM) | V max (µmol min−1 mg−1) | k cat (s−1) | k cat/K m (s−1 mM −1) |
---|---|---|---|---|
Wild type | 17 ± 4 | 0.9 ± 0.02 | 0.55 ± 0.04 | 33 |
T89S | 4 ± 2.1 | 0.2 ± 0.03 | 0.12 ± 0.02 | 31 |